Research Paper Volume 12, Issue 4 pp 3298—3311

Figure 1. Effects of BBR on the survival and apoptosis of ALL cells. ALL EU-4 and EU-6 cells were treated with BBR (0, 1, 10, 50, 100, 200 μM) for 24 h. (A–B) Cell viability was analyzed by the CCK-8 assay. (C–D) Cell apoptosis was detected by TUNEL assay and the percentage of apoptotic cells was summarized. (E–F) Representative protein expression of the cleaved caspase-3 and pro-caspase-3 in cells treated with BBR at the indicated concentrations. Numerical label above each protein band indicates the fold change relative to expression of caspase-3 in the cells without BBR treatment. (G–H) Caspase-3 activity in the cells with BBR at the indicated concentrations was determined using the Caspase-3 Colorimetric Assay Kit. (I–J) ALL cells EU-4 and EU-6 cells were incubated 24 h with 50 μM BBR in the presence or absence of z-VAD-FMK (50 μM). Cell apoptosis was measured by TUNEL assay. Results are expressed as mean ± SD **p<0.01, ***p<0.001, compared to control group; *p<0.05, **p<0.01, ***p<0.001, compared to indicated group.