Figure 5. MiR-24-3p is implicated in the BBR-induced apoptosis by targeting PIM-2. (A) The expression profile of miR-24-3p in ALL cells, KOPN-8, EU-4, NALM-6, EU-6, SEM, and human lymphoma cell H9. (B) Effect of BBR on miR-24-3p expression in EU-4 and EU-6 cells. EU-4 and EU-6 cells were treated by BBR at the indicated concentrations for 24 h. The relative expression of miR-24-3p was measured by qRT-PCR and normalized to U6. EU-4 and EU-6 cells were transfected with siRNA targeting miR-24-3p (simiR-24-3p) or negative control (siControl), and subsequently treated with 50 μM BBR for 24 h. (C) Cell apoptosis was measured by TUNEL assay. (D) Representative western blot analysis showing PIM-2 expression in the cells. (E) Representative images of PIM-2 immunofluorescence staining in the treated cells. The numbers above the western blots band are the quantification of the gray value normalized to control group. Results are expressed as mean ± SD. *p<0.05, **p<0.01, ***p<0.001, compared to the indicated group.