Research Paper Volume 12, Issue 4 pp 3298—3311

Berberine promotes XIAP-mediated cells apoptosis by upregulation of miR-24-3p in acute lymphoblastic leukemia

Figure 6. BBR extends the survival and attenuates the pathological conditions in ALL-transplanted xenograft mice. SCID mice, injected with either EU-4 cells or an equivalent volume of PBS via tail vein, were treated with BBR at 1 or 10 mg/kg (n=6 mice per group). (A) Kaplan-Meier survival curves showing the mortality of the mice. (B) Donor-derived ALL cells were analyzed by flow cytometry. (C) Representative images of H&E staining of liver tissues (magnification×). (D) The levels of alanine aminotransferase (ALT) and (E) aspartate aminotransferase (AST) in serum were measured. The number of (F) white blood cells (WBC) and (G) red blood cells (RBC) in blood. (H) Serum levels of hemoglobin. SCID mice, injected with wild-type (WT) or XIAP knockout EU-4 cells (XIAP-/-) via tail vein, were treated with 10 mg/kg BBR (n=6 mice per group). Results are expressed as mean ± SD. ***p<0.001, compared to the Control; #p<0.05, ##p<0.01, compared to ALL group.