Figure 8. Validation of HIF-1 related genes and reliability of risk score in the validation cohort. There were significant correlations between HIF-1 related genes (A). Among the four selected genes in the derivation cohort, ENO1, GAPDH, and SLC2A1 were upregulated in HCC (B). PCA and t-SNE analysis confirmed the clustering ability of the four-gene panel in the validation cohort (C, D). Time-dependent ROC analysis showed the diagnostic value of risk score for overall survival at 5 years after diagnosis (E).