Research Paper Volume 12, Issue 4 pp 3617—3625

Inflammatory macrophages facilitate mechanical stress-induced osteogenesis

Figure 4. Saporin-CD11b reduces macrophages increase during DO. (A, B) RT-qPCR for CD68 mRNA (A) and ELISA for CD68 protein (B) in regenerating bone tissue from saporin-CD11b-treated mice or control IgG-treated mice. (C, D) FACS for CD68+ cells in regenerating bone tissue saporin-CD11b-treated mice or control IgG-treated mice by representative flow charts (C), and by quantification (D). (EI) ELISA for IL-1β (E), IL-6 (F), TNFα (G), BMP2 (H) and TGFβ (I) from FAC-sorted CD68+ macrophages. DP-day 0: day 0 of distraction phase, CP-day 0: day 0 of consolidation phase, CP-day 28: day 28 or consolidation phase. *p<0.05. NS: non-significant. N=8.