Research Paper Volume 12, Issue 4 pp 3626—3646

NMR-based metabonomic analysis of HUVEC cells during replicative senescence

Figure 5. Significantly altered metabolic pathways in the four groups of HUVEC cells compared to the P3 group. (A) P6 vs. P3; (B) P10 vs. P3; (C) P14 vs. P3; (D) P18 vs. P3. A bubble represents an identified metabolic pathway. The bubble size is proportional to the pathway impact value (PIV), with the color denoting the statistical significance [-ln(p)] from highest (in red) to lowest (in white). Metabolic pathways with -ln(p) > 8 and PIV > 0.03 were identified to be significantly altered metabolic pathways, including: a, glycerophospholipid metabolism; b, pantothenate and CoA biosynthesis; c, inositol phosphate metabolism; d, pyruvate metabolism; e, glycine, serine and threonine metabolism; f, taurine and hypotaurine metabolism; g, aminoacyl-tRNA biosynthesis; h, propanoate metabolism; I, purine metabolism; j, β-Alanine metabolism; k, glanine, aspartate and glutamate metabolism; l, arginine and proline metabolism; m, glutathione metabolism; n, glutamate and glutamine metabolism.