Research Paper Volume 12, Issue 4 pp 3713—3729

Identification of a lathyrane-type diterpenoid EM-E-11-4 as a novel paclitaxel resistance reversing agent with multiple mechanisms of action

Figure 7. EM-E-11-4 inhibited the expression of βIII-tubulin. (A) Hela/βIII cells were treated with EM-E-11-4 (2.5, 5, and 10 μM) for 48 h, and the expression of βIII-tubulin was determined by Western blot analysis. Columns represent the means±SD values for protein levels obtained from three individual experiments. * p<0.05 and ** p<0.01 vs. control (EM-E-11-4 0μM). (B) Immunofluorescence analysis (×600). Blue: nucleus; green: βIII-tubulin; red: β-tubulin. There is no clear green fluorescence in Hela cells, whereas Hela/βIII cells have a clear green fluorescence. After Hela/βIII cells were treated with EM-E-11-4 48 h, the green fluorescence was weakened.