Figure 6. Baicalin remodels gut microbiota in repeated cerebral ischemia-reperfusion model mice. (A) Metagenomic assay analysis of the stool samples to determine the phyla of gut microbiota in control, model, and Baicalin-treated model group mice. (B) The most abundant gut microbial species levels in control, model, and baicalin-treated model group mice, as analyzed by linear discriminant analysis (LDA) coupled with effect size measurements are shown. The enriched taxa in the model and control group mice are indicated based on negative (orange) and positive (gray) scores, respectively. Also shown is the significant threshold LDA value of >3. (C) Heat map of the metagenomic analyses of gut microbial species in the stool samples of control and model group mice are shown. The red and green color codes represent high and low values, respectively. (D) Principal component analysis (PCA) scores of the gut microbial species in the stool samples of control and model group mice are shown. (E) Spearman correlation analysis shows the association between PCA scores and the behavioral profile, brain FC strength, and hippocampal neuron spasticity in control and model group mice. Note: * denotes P<0.05 compared with the model mice using one-way ANOVA analysis followed by Dunnett`s post hoc test. All the values are expressed as means ± S.D. Each group had 15 mice (n=15).