Research Paper Volume 12, Issue 4 pp 3828—3847

Ancestral stress programs sex-specific biological aging trajectories and non-communicable disease risk

Figure 7. MPS rat lineages and experimental design. (A) The MPS lineage was generated by stressing pregnant dams over four consecutive generations (F0, F1, F2, F3) to produce multigenerationally stressed (MPS) F4 offspring. Yoked non-stress CONTROL F4 offspring were generated in parallel. (B) Behavioural phenotype was assessed in open field exploratory behaviour, forced swim task learned helplessness, and ladder rung skilled walking. (C) MPS and CONTROL F4 generations were used for a mixed longitudinal experiment with tests at 6, 12 and 18 months of age.