Research Paper Volume 12, Issue 4 pp 3848—3861

Establishment and characterization of patient-derived xenografts for hormone-naïve and castrate-resistant prostate cancers to improve treatment modality evaluation

Figure 1. Establishment of molecular heterogeneous PDX models with maintained pathological features of patient tumors. (A) NIRF optical imaging of NPG mice with PC xenograft tumors using MHI-148 dye (Top). The PDX models of PC were established by implantation of clinical tumor specimens D17225 and B45353 into the renal capsule of NPG mice. Gross morphology of PC tissue-implanted mouse kidney (bottom). (B, C) H&E and IHC analyses of tumor tissues derived from both PDX models and patient samples (D17225 and B45353). (D) Multiple organ metastasis PDX models from B45354 (CRPC patient). H&E staining of metastatic tumors (including the lung and liver). Original magnification, 400×; scale bars represent 20 μm.