Figure 4. Ca2+ sparks activity was increased by caffeine and was decreased by ryanodine in both unafflicted control and DO myocyte treatment groups. (A, B) Representative Ca2+ sparks recorded in control (A) and DO (B) detrusor myocytes before and after exposure to10 μM caffeine (Ca2+ spark activator) (b) and before and after exposure to 10 μM ryanodine (Ca2+ spark inhibitor) (c). (C–G) provide summary data for Ca2+ spark frequency, F/F0, FWHM, rise time, and half-time decay of the control and DO destrusor unafflicted samples for treatments with caffeine (10 μM) and ryanodine (10 μM). To record the Ca2+ sparks, we clamped detrusor myocytes at -40 mV. We used one-way ANOVA for comparisons between groups. VS control, *P < 0.05, and **P < 0.01; VS DO, Δ P < 0.05, and ΔΔ P < 0.01.