Research Paper Volume 12, Issue 4 pp 3880—3898

Adipose-derived mesenchymal stem cells-derived exosome-mediated microRNA-342-5p protects endothelial cells against atherosclerosis

Figure 5. ADSCs-derived exosome was characterized and constrained the expression of miR-342-5p in HUVECs impaired by H2O2. (A) The spindle-like ADSCs were observed through the microscope at a magnification of ×40. (B) TEM analysis presented that exosomes were round membrane-bound vesicles with 30 to 150 nm diameter. Scale bar indicates 50 nm. (C) The common marker proteins for exosomes, CD9, CD63 and TGS101, were determined by western blot assay. (D) The expression level of miR-342-5p was subsequently detected by qRT-PCR assay after added the obtained exosomes at a mass of 10 ug or 20 ug into HUVECs impaired by H2O2 at a concentration of 1500 uM. *, P<0.05, ***, P<0.001. ADSCs, adipose-derived mesenchymal stem cells; miR-342-5p, microRNA-342-5p; HUVECs, human umbilical vein endothelial cells; qRT-PCR, quantitative real-time polymerase chain reaction; PBS, phosphate-buffered saline.