Figure 2. circNT5E silencing inhibits NSCLC cell growth, proliferation and migration. The stable A549 cells (A–D) or the primary human NSCLC cells (Pri-Ca-1/-2/-3, E-H) with the lentivirus-packaged circNT5E shRNA (“sh-circNT5E-Seq-1/2”, two different sequences) or the non-sense control shRNA (“sh-c”) were cultured, the circNT5E expression was tested by qPCR (A and E), cell growth (cell counting assay, B and F), proliferation (EdU incorporation, C and G) and migration (“Transwell” assay, D and H) were tested by the mentioned assays; “Pare” stands for the parental control cells (Same for all Figures). Error bars stand for mean ± standard deviation (SD, n=5). * P <0.05 vs. “Pare”/“sh-c” cells. Experiments in this figure were repeated five times, and similar results were obtained. Bar= 100 μm (C, D, G and H). For all the functional assays exact same number of viable NSCLC cells with applied genetic treatments were initially seeded into each well/dish (at 0h), and cells cultured for applied time periods (Same for all Figures).