Figure 2. Distribution of SIRT3 in cytoplasm and nuclear of cardiomyocytes. In H9c2 cells, Ad-GFP or Ad-SIRT3 were transfected, or 10 μM ISO stimulated for 3-24 h. Total, cytoplasm and nuclear fraction of protein were extracted, and the protein expressions of SIRT3 were detected in total (A), cytoplasm (B) and nuclear (C) by western blot. Data were presented as means±SE. *P<0.05 versus control group, n=4 independent experiments.