Figure 4. Distribution of SIRT3 in cytoplasm and nuclear of SD rat heart tissues. SD rats of ISO group were subjected to subcutaneous injections of 1.5 mg/kg/d isoproterenol for 7 d. The control group was given the same dose of saline. Total, cytoplasm and nuclear fraction of protein were extracted, and the protein expressions of SIRT3 were detected in total (A), cytoplasm (B) and nuclear (C) by western blot. Data were presented as means±SE. *P<0.05 versus control group, n=4 independent experiments.