Figure 5. ISO upregulates PARP-1 activity. H9c2 cells were treated with 10 μM ISO for 3-24 h and SD rats were subjected to subcutaneous injections of 1.5 mg/kg/d isoproterenol for 7 d. The H9c2 cells protein (A) and SD rats’ heart tissues protein (B) were extracted after the above treatment. Western blot was used to detect PARP-1 activity. Data were presented as means±SE. *P<0.05 versus CON or NS group, n=4 independent experiments.