Research Paper Volume 12, Issue 5 pp 4178—4192

SIRT3 inhibits cardiac hypertrophy by regulating PARP-1 activity

Figure 6. SIRT3 overexpression inhibits ISO or PE-induced upregulation of PARP-1 activity. In H9c2 cells, Ad-GFP or Ad-SIRT3 were transfected and then stimulated with 10 μM ISO or 100 μM PE for 24 h. Western blot was used to detect PARP-1, SIRT3 protein expression and PARP-1 activity. Data were presented as means±SE. *P<0.05 versus GFP group, #P<0.05 versus GFP treated with ISO or PE, n=4 independent experiments.