Figure 7. SIRT3 interacts with and deacetylates PARP-1 in cardiomyocytes. In H9c2 cells, Ad-GFP or Ad-SIRT3 were transfected and then stimulated with 10 μM ISO for 24 h. PARP-1 was precipitated using PARP-1 antibody and detected with acetylated antibody, PARP-1 antibody, and SIRT3 antibody (A). In H9c2 cells, nuclear protein was extracted after 24 h stimulation with ISO. Nuclear SIRT3 protein was precipitated with SIRT3 antibody, and corresponding protein expression was detected with PARP-1 antibody and SIRT3 antibody (B). Data were presented as means±SE. *P<0.05 versus GFP group, #P<0.05 versus GFP treated with ISO, n=4 independent experiments. Images representative of four independent experiments are shown.