Figure 8. SIRT3 interacts with PARP-1 in heart tissues. SD rats of ISO group were subjected to subcutaneous injections of 1.5 mg/kg/d isoproterenol for 7 d. The control group (NS) was given the same dose of saline. Nuclear protein of heart tissues was extracted. PARP-1 was precipitated using PARP-1 antibody and detected with acetylated antibody, PARP-1 antibody, and SIRT3 antibody (A). NAD+ content in heart tissues of SD rats was detected by NAD+/NADH detection kit (B). Images representative of four independent experiments are shown. Data were presented as means±SE. *P<0.05 versus NS group, n=4 independent experiments.