Figure 4. Depletion of TOP2B in mouse matured oocytes led to early embryo arrest at the 2-cell stage. (A) TOP2B knockdown efficiency in young mouse MII oocytes was assessed by qRT-PCR. Paired two-tailed Student’s t-test. (B) Representative images of mouse early embryos knocking down TOP2B mRNA. Bars = 100μm. (C) Knocking down TOP2B significantly reduces the developmental rate of early embryos (n=35,35,35). Paired two-tailed Student’s t-test.