Research Paper Volume 12, Issue 4 pp 3950—3961

Advanced maternal age alters expression of maternal effect genes that are essential for human oocyte quality

Figure 4. Depletion of TOP2B in mouse matured oocytes led to early embryo arrest at the 2-cell stage. (A) TOP2B knockdown efficiency in young mouse MII oocytes was assessed by qRT-PCR. Paired two-tailed Student’s t-test. (B) Representative images of mouse early embryos knocking down TOP2B mRNA. Bars = 100μm. (C) Knocking down TOP2B significantly reduces the developmental rate of early embryos (n=35,35,35). Paired two-tailed Student’s t-test.