Research Paper Volume 12, Issue 4 pp 3962—3975

SOCS1 blocks G1-S transition in hepatocellular carcinoma by reducing the stability of the CyclinD1/CDK4 complex in the nucleus

Figure 1. SOCS1 expression levels in HCC and normal tissues. (A) Oncomine-Online Platform (www.oncomine.org) showed reduced SOCS1 expression in HCC and paired normal liver tissues. ***P <0.001, ****P <0.0001. (B, C) In 159 pairs of tissues, SOCS1 expression was apparently downregulated in tumours compared with adjacent liver tissues. ****P <0.0001. In the majority of HCC tissues (71%), SOCS1 mRNA levels were reduced. (D) Immunohistochemical analysis of SOCS1 expression in 90 paired HCC (Odd number) and normal liver tissues (Even number). (E) The level of SOCS1 mRNA and protein expression was reduced in SMMC-7721, MHCC-97H, Hep3B, HepG2, Huh-7, HCCLM3 and SK-Hep-1 cells. ** P <0.005, ***P <0.001, ****P <0.0001. (F) The level of SOCS1 methylation increased in most HCC cell lines. (G) SMMC-7721, HCC-LM3 and MHCC-97H cells showed successful stable overexpression of SOCS1.