Research Paper Volume 12, Issue 4 pp 3962—3975

SOCS1 blocks G1-S transition in hepatocellular carcinoma by reducing the stability of the CyclinD1/CDK4 complex in the nucleus

Figure 2. SOCS1 inhibited HCC cell proliferation in vitro and in vivo. (A) Cell viability was analysed by the CCK-8 assay. ****P <0.0001. (B) Representative FACS images of SMMC-7721, HCC-LM3 and MHCC-97H cells infected with LV-GFP or LV-SOCS1. * P <0.05, ** P <0.005. (C) Representative images of EdU incorporation assays in SMMC-7721 and HCC-LM3 cells. * P <0.05. (D) LV-SOCS1-transfected SMMC-7721 cells exhibited significantly reduced tumour volume and weight compared to those in control cells. ***P <0.001.