Research Paper Volume 12, Issue 4 pp 3976—3992

PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission

Figure 1. Verifying changes in PKM2 expression when treated with DASA-58 or C3k. (A, B) BMSCs were cultured with different concentration of DASA-58 (0, 5, 10, 30, 50 μM) or C3k (0, 0.05, 0.15, 0.3, 0.5 μM) for 1, 3 and 5 days. BMSCs viability was assessed by CCK-8 assays. (C) After 4 days culturing with DASA-58 (30μM) or C3k (0.15 μM), PKM2 immunofluorescence staining on BMSCs was conducted. (D, E) BMSCs were treated with DASA-58 (30μM) or C3k (0.15 μM) for 7 days. Total proteins, nuclear proteins and cytoplasm proteins were extracted, then total PKM2, nucl-PKM2 and cyto-PKM2 were measured by western blot and quantified. All the experiments have been repeated independently at least 3 times. Data are represented as mean ± SD. *P < 0.05 versus control group.