Research Paper Volume 12, Issue 4 pp 3976—3992

Figure 2. Impact of PKM2 on osteogenic differentiation of BMSCs. (A) BMSCs were cultured in osteogenic medium with or without DASA-58 (30μM) or C3k (0.15 μM). 7 days later, ALP staining detection was performed. (B) Alizarin red staining was performed after 14 days of treatment, and (C) ALP activity was measured after 7 days of treatment. (D) The relative expression of ALP, COL1, OPN and RUNX2 mRNA levels to GAPDH were detected on 7^th day after the induction of basic or osteogenic medium in the presence or absence of DASA-58 (30μM) or C3k (0.15 μM). All the experiments were repeated independently at least 3 times. Data are represented as mean ± SD. *P < 0.05, **P < 0.01 versus control group.