Figure 3. Inhibition of PKM2 promoted osteogenesis and upregulated the β-catenin signaling pathway. (A–C) BMSCs were cultured in osteogenic medium with or without 30 μM DASA-58 or 0.15 μM C3k for 7 days. Protein levels of RUNX2 and OPN were measured by western blot, and immunofluorescence staining for RUNX2 was performed. (D, E) BMSCs were treated with DASA-58 and C3k for 0h, 12h, 24h and 48h respectively. Protein level of active-β-catenin was detected with western blot. (F) BMSCs were cultured with osteogenic medium with or without DASA-58 or C3k for 7 days, then active-β-catenin immunofluorescence staining was performed. All the experiments were repeated independently at least 3 times. Data are represented as mean ± SD. *P < 0.05, **P < 0.01.