Research Paper Volume 12, Issue 4 pp 3976—3992

PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission

Figure 6. DASA-58 reduced mitochondrial membrane potential (MMP) of BMSCs during osteogenic differentiation, C3k rose it. BMSCs were cultured in osteogenic medium added with or without 30 μM DASA-58 or 0.15 μM C3k for 2 and 4 days, (A, B) then JC-1 staining was performed. Red fluorescence represents JC-1 aggregation in healthy mitochondria, while green fluorescence represents cytosolic JC-1 monomers manifesting MMP collapse. Merged images indicated co-localization of JC-1 aggregates and monomers. (C, D) JC-1 monomer percentage was shown. (E) The flow cytometric analysis was conducted after the cells were stained by JC-1 (FL1 represents JC-1 green and FL2 represents JC-1 red). All the experiments have been repeated independently at least 3 times. Data are represented as mean ± SD. *P < 0.05 and **P < 0.01.