Research Paper Volume 12, Issue 4 pp 3976—3992

PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission

Figure 7. Effects of PKM2 on adipogenic differentiation of BMSCs. (A, B) BMSCs were treated with or without 30 μM DASA-58 or 0.15 μM C3k in adipogenic medium for 14 days, then cells were stained with Oil Red O solution. (C, D) BMSCs were cultured with basic medium or adipogenic medium supplemented with or without the same concentration of DASA-58 or C3k. 7 days later, mRNA levels of Adipsin, FABP4 and PPARγ were quantified by qRT-PCR. (EH) BMSCs were as described above for 7 days, protein levels of PPARγ and FABP4 were detected by western blot, and PPARγ immunofluorescence staining was performed. (I, J) BMSCs were treated as described above for 0h, 12h, 24h and 48h respectively. Protein expression of active-β-catenin was measured by western blot. All the experiments were repeated at least 3 times independently. Scale bar represents 400 μm. Data are represented as mean ± SD. *P < 0.05 and **P < 0.01.