Research Paper Volume 12, Issue 10 pp 8820—8836

LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/β-catenin signaling pathway

Figure 2. Overexpressed HPRT1 inhibits dopaminergic neuron loss in 6-OHDA-induced PD mice. 6-OHDA-induced PD mice were treated with oe-NC or oe-HPRT1. (A) Protein expression of HPRT1 in the substantia nigra tissues examined by western blot assay. (B) TH positive neurons in the substantia nigra examined by immunohistochemistry (upper × 100, lower × 400). (C) Fluoro-Jade B-stained apoptotic neurons (scale bar = 50 μm). (DG) The mRNA expression of Nurr-1 (D), Pitx-3 (E), Ngn-2 (F) and NeuroD1 (G) in the substantia nigra tissues examined by RT-qPCR. *p < 0.05. n = 6. Measurement data are by means ± standard deviation. Comparison between two groups was analyzed by independent sample t test.