Figure 3. Overexpressed HPRT1 activates the Wnt/β-catenin signaling pathway in a PD model. 6-OHDA-treated N27 dopaminergic neurons were treated with oe-NC or oe-HPRT1. (A) The protein expression of total-β-catenin and DAT as well as the extent of β-catenin phosphorylation in the N27 dopaminergic neurons detected by western blot assay. (B) The activity of the Wnt/β-catenin signaling pathway expressed by TOP/FOP ratio using TOP/FOP flash reporter assay. FOP was designated as background value or as negative control due to its stability. *p < 0.05. Measurement data from three independent experiments are expressed by means ± standard deviation. Comparison between two groups was analyzed by unpaired t test.