Figure 2. Expression of VEGF/Notch pathway-related proteins and CD31 in rat lung tissues. (A) Immunohistochemical staining of Notch1, CD31, and VEGFR2 (400X), and VEGF-A and Hes-1 (200X). (B) Western blotting analysis of Hes-1, Notch1, VEGF-A and VEGFR2 expression in rat lung tissue. Data are mean ± SEM. *P<0.05, **P<0.01 compared with the normoxic control group; #P<0.05, ##P<0.01 compared with the 24-h hypoxia group. &&P<0.01 compared with the 48-h hypoxia group.