Figure 4. Analysis of hypoxia-induced changes in VEGF/Notch signaling effector molecules and related miRNAs in PMVECs. (A) PMVECs were cultured under hypoxia for 0, 24, 48, or 72 h. With prolongation of hypoxic exposure the cells lost their original morphology and cell population density decreased (200X). (B) Relative expression of miR-30b-5p, miR-532-5p, miR-203a-3p, miR-101a-5p, and miR-16-3p assessed by qRT-PCR. (C) Relative expression of Hes-1, Notch1, VEGF-A, and VEGFR2 mRNA measured by qRT-PCR. (D) Hes-1, Notch1, VEGF-A, and VEGFR2 expression assessed by western blotting. Data are mean ± SEM. *P < 0.05, **P < 0.01 compared with the normoxic control group; #P < 0.05, ##P < 0.01 compared with the 24-h hypoxia group; &P < 0.05, &&P < 0.01 compared with the 48-h hypoxia group.