Research Paper Volume 12, Issue 5 pp 4247—4267

Downregulation of lung miR-203a-3p expression by high-altitude hypoxia enhances VEGF/Notch signaling

Figure 5. miR-203a-3p mimics expression inhibits the expression of VEGF-A and downstream genes. (A) Strong GFP fluorescence in PMVECs transduced with miR-203a-3p mimics indicated significantly increased expression of miR-203a-3p, compared with the corresponding control (no transduction) (P < 0.01) (100X). (B) Morphological changes in PMVECs transduced with lentiviral vectors encoding miR-203a-3p-NC (negative miRNA-203a-3p mimics control) or miR-203a-mimics (100X). (C) Results of qRT-PCR analysis showing decreased expression of Hes-1, VEGF-A, and VEGFR2 in miR-203a-mimics-transduced PMVECs (P < 0.01). (D) Western blotting results showing reduction on Hes-1, VEGF-A and VEGFR2 expression in PMVECs transduced with miR-203a-3p-mimics. Data are mean ± SEM. **P < 0.01 compared with the control or normoxic control groups; ##P < 0.01 compared with miR-203a-3p-NC or 24-h hypoxia group; &P < 0.05, &&P < 0.01 compared with the 48-h hypoxia group.