Research Paper Volume 12, Issue 5 pp 4247—4267

Downregulation of lung miR-203a-3p expression by high-altitude hypoxia enhances VEGF/Notch signaling

Figure 6. miR-203a-3p mimics expression decreases survival and angiogenic activity and induces a pro-inflammatory response in PMVECs. (A) Apoptosis assay results showing increased apoptosis rate in PMVECs expressing miR-203a-3p mimics. (B) In vitro angiogenesis assay results. PMVECs transduced with miR-203a-3p mimics showed weak angiogenic ability, which improved however with prolonged hypoxic incubation time. (C) ELISA assay results showing increased IL-6, IL-10, and TNF-α secretion in PMVECs transfected with miR-203a-3p. (D, E) CCK8 assay results indicating decreased survival rate in PMVECs transfected with miR-203a-3p mimics. Data are mean ± SEM. *P < 0.05, **P < 0.01 compared with the control (no transduction) or normoxic control groups; #P < 0.05, ##P < 0.01 compared with the miR-203a-3p-NC (negative miRNA-203a-3p mimics control) or 24-h hypoxia groups; &P < 0.05, &&P < 0.01 compared with the 48-h hypoxia group.