Research Paper Volume 12, Issue 5 pp 4247—4267

Downregulation of lung miR-203a-3p expression by high-altitude hypoxia enhances VEGF/Notch signaling

Figure 7. VEGF-A is a direct target of miR-203a-3p. (A) Sequence information of miR-203 binding sites in the 3′-UTR region of the VEGF-A mRNA. (B) Dual luciferase assay indicating decreased fluorescence in PMVECs co-expressing pmirGLO/VEGF-UTR and miR-203-3p mimics. (C) Western blotting detection of target genes of miR-203a-3p. Compared with mimics-NC (negative miR-203a-3p mimics control), VEGF expression decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. (D) qRT-PCR detection of target genes of miR-203a-3p. VEGF mRNA levels decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. Data are mean ± SEM. *P < 0.05, **P < 0.01 compared with mimics-NC; ##P < 0.01 compared with miR-203a-3p-mimics; &&P < 0.01 compared with miR-203a-3p-ASO-NC (negative miR-203a-3p-ASO control).