**Figure 2.** **Transient global cerebral ischemia-reperfusion aggravates NMDAR-dependent spine dysfunction.** (**A, B**) Top, layer 1 (30~50 um) spines images on the 3rd day after reperfusion, where the red circle represents the selected spine in Reper (**A**) and Ro25-6981-treated (**B**) mice. Bottom, the traces of selected spines. (**C, D**) Superimposed traces from the shaded areas in a and b. Each color represents a different cell. (**E, F**) The overall trend of the average frequency of Ca^{2+} transients before and after 6 hours (**E**) and 28 days (**F**) of ischemia-reperfusion. (**G**) The average frequency of Ca^{2+} transients in Reper and Ro25-698-treated mice. The 3rd hour (left, n= 438 spines in 6 Reper mice, n= 312 spines in 4 Ro25-698-treated mice), the 3rd day (middle, n= 432 spines in 6 Reper mice, n= 328 spines in 4 Ro25-698-treated mice) and the 14^{th} day (right, n=436 spines in 6 Reper mice, n= 329 spines in 4 Ro25-6981-treated mice). (**H**) The fractions of hyperactive spines Reper mice (n= 432 spines) and Ro25-698-treated mice (n=328 spines) on the 3rd day after reperfusion. **P* < 0.05, ns, not significant, Student’s t-test. Error bars = s.e.m.