Figure 4. Sirt1 is involved in the anti-fibrosis function of lncRNA sirt1 antisense (AS). (A–E) Western blot assay and quantitative analyses of sirt1 protein levels in RLE-6TN cells transfected with ad-NC, ad-sirt1 AS, ad-sirt1 AS+si-NC, ad-sirt1 AS+si-sirt1 under TGF-β1 treatment. (F) Immunofluorescence staining showing the overlap of E-cadherin (red) and α-SMA (green) in pulmonary tissues from mice intratracheally infected with ad-NC, ad-sirt1 AS, ad-sirt1 AS+ad-si-NC, ad-sirt1 AS+ad-si-sirt1. (G) H&E and Masson’s trichrome staining showed pulmonary injury and collagen deposition of pulmonary tissues in mice with above indicated treatment. * P<0.05 vs. control; ** P<0.05 vs. TGF-β1+ad-NC; # P<0.05 vs. TGF-β1+ad-sirt1 Ad+si-NC.