Research Paper Volume 12, Issue 5 pp 4474—4488

Irisin activates Opa1-induced mitophagy to protect cardiomyocytes against apoptosis following myocardial infarction

Figure 4. Opa1-induced mitophagy maintains mitochondrial function and reduces oxidative stress. (A, B) Analysis of ROS levels in cardiomyocytes. (CE) ELISA assays to evaluate the levels of antioxidants. (F, G) Measurement of alterations in the mitochondrial membrane potential using a JC-1 probe. *P < 0.05 vs. the control group; #P < 0.05 vs. the hypoxia + irisin group.