Figure 4. Opa1-induced mitophagy maintains mitochondrial function and reduces oxidative stress. (A, B) Analysis of ROS levels in cardiomyocytes. (C–E) ELISA assays to evaluate the levels of antioxidants. (F, G) Measurement of alterations in the mitochondrial membrane potential using a JC-1 probe. *P < 0.05 vs. the control group; #P < 0.05 vs. the hypoxia + irisin group.