Figure 5. Opa1-induced mitophagy inhibits the mitochondrial apoptosis pathway. (A) Analysis of caspase-3 activity in cardiomyocytes by ELISA. (B, C) Western blot analysis of cyt-c levels in the cytoplasm and mitochondria. (D–F) Immunofluorescence analysis of Bax and caspase-9 expression in cardiomyocytes. *P < 0.05 vs. the control group; #P < 0.05 vs. the hypoxia + irisin group.