Research Paper Volume 12, Issue 5 pp 4489—4505

Methionine abrogates the renoprotective effect of a low-protein diet against diabetic kidney disease in obese rats with type 2 diabetes

Figure 4. Changes in the renal Gnmt expression and SAM content. Representative western blots of Gnmt and β-actin in the renal cortex (A). Quantitative ratios of Gnmt to β-actin (B) and mRNA expression of Gnmt adjusted to 18S levels (C) in the renal cortex (n=7). Representative photographs of SAM immunohistochemistry of the kidney (scale bar: 100 μm) (D). Semiquantitation of the SAM staining scores (E) (n=3). SAM levels in the renal cortex (F) (n=3). Representative western blots of LCMT1, methylated-PP2A, PP2A and β-actin in the renal cortex (G). Quantitative ratios of LCMT1 to β-actin (H) and methylated-PP2A to PP2A (I) in the renal cortex (n=7). The data shown are the means ± SD. **p<0.01, ***p<0.001 vs. the indicated groups. ns: not significant. Gnmt: glycine-N-methyltransferase, SAM: S-adenosylmethionine, LCMT1: leucine carboxyl methyltransferase 1, PP2A: protein phosphatase 2A.