Research Paper Volume 12, Issue 5 pp 4489—4505

Methionine abrogates the renoprotective effect of a low-protein diet against diabetic kidney disease in obese rats with type 2 diabetes

Figure 5. Changes in mTORC1 activity in the kidney. Representative photographs of immunohistochemistry for p-S6RP in tubular lesions (A) (scale bar: 100 μm). Semiquantitation of p-S6RP staining scores in tubulointerstitial lesions (B) (n=3). Representative western blots of p-S6RP and S6RP in the renal cortex (C). Quantitative ratios of p-S6RP to S6RP (D) (n=7). The data shown are the means ± SD. *p<0.05, ***p<0.001 vs. the indicated groups. ns: not significant. mTORC1: mechanistic target of rapamycin complex 1, S6RP: phospho-S6 ribosomal protein.