Research Paper Volume 12, Issue 5 pp 4489—4505

Methionine abrogates the renoprotective effect of a low-protein diet against diabetic kidney disease in obese rats with type 2 diabetes

Figure 7. Effect of SAM on amino acid-free-induced autophagy and methyl-PP2A and p-S6RP expression in cultured HK-2 cells and change of starvation-induced autophagy in the renal cortex of Gnmt knockout (KO) mice. Representative western blots of LC3 in HK-2 cells (A). Quantitative autophagic flux in cultured HK-2 cells (B) (n=3). Representative western blots of LC3 in the renal cortex of Gnmt KO mice after 48 hours of starvation (C). Quantitative autophagic flux in the renal cortex of mice (D) (n=3). Representative western blots of methyl-PP2A, PP2A, p-S6RP and S6RP in HK-2 cells (E). Quantitative expression of methyl-PP2A to PP2A (F) and p-S6RP to S6RP (G) in HK-2 cells (n=3). The data shown are the means ± SD. *p<0.05, ***p<0.001 vs. the indicated groups. ns: not significant. SAM: S-adenosylmethionine, Gnmt: glycine-N-methyltransferase, CQ: chloroquine, LC3: Microtubule-associated protein 1 light chain 3, WT: wild type, Gnmt: glycine N-methyltransferase, SAM: S-adenosylmethionine, Methyl-PP2A: methylated protein phosphatase 2A, PP2A: protein phosphatase 2A, p-S6RP: phospho-S6 ribosomal protein, S6RP: S6 ribosomal protein.