Research Paper Volume 12, Issue 5 pp 4527—4546

LncRNA-LALR1 upregulates small nucleolar RNA SNORD72 to promote growth and invasion of hepatocellular carcinoma

Figure 5. LncRNA-LALR1 upregulates SNORD72 and ID2 in HCC cells. (A) qRT-PCR showing the expression of SNORD72 and ID2 after lncRNA-LALR1 silencing in SMMC-7721 cells. (B) Western blot showing the expression of ID2 after lncRNA-LALR1 silencing in SMMC-7721 cells. (C) qRT-PCR showing the expression of SNORD72 and ID2 after knockdown of lncRNA-LALR1 in HepG2 cells. (D) Western blot showing the expression of ID2 after lncRNA-LALR1 silencing in HepG2 cells. (E) FISH assay showing the expression of SNORD72 after lncRNA-LALR1 silencing in SMMC-7721 cells. (F) FISH assay showing the expression of ID2 after lncRNA-LALR1 silencing in SMMC-7721 cells. (G) FISH showing lncRNA-LALR1 is co-localized with SNORD72 in HepG2, SMMC-7721, PLC/PRF/5, Sk-Hep1 cells. (H) RNA pulldown assay showing lncRNA-LALR1 interacts with SNORD72 and ID2 mRNA. (I) The effect of lncRNA-LALR1 on the mRNA stability of ID2. (J) The effect of lncRNA-LALR1 on the mRNA stability of SNORD72. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ns: no significant.