Research Paper Volume 12, Issue 5 pp 4527—4546

LncRNA-LALR1 upregulates small nucleolar RNA SNORD72 to promote growth and invasion of hepatocellular carcinoma

Figure 6. SNORD72 is upregulated in HCC samples and promotes proliferation and invasion of HCC cells. (A) Bioinformatic analysis showing the expression of SNORD72 in HCC tissues and adjacent non-tumor tissues. (B) qRT-PCR assay showing the expression of SNORD72 in HCC tissues and matched non-tumor tissues. (C) Bioinformatic analysis showing the relationship between SNORD72 expression and the differentiation degree. (D) qRT-PCR assay verifying the overexpression of SNORD72. (E) FISH assay verifying the overexpression of SNORD72. (F) CCK-8 assay showing overexpression of SNORD72 increases the proliferation ability compared with control group in vitro. (G) Colony formation assay showing overexpression of SNORD72 increases the growth ability compared with control group in vitro. (H) Transwell invasion assay showing overexpression of SNORD72 increases the invasion ability compared with control group in vitro. (I) qRT-PCR assay showing the effect of SNORD72 on the expression of ID2. (J) Western blot assay showing the effect of SNORD72 on the expression of ID2. (K) FISH assay showing the effect of SNORD72 on the expression of ID2. (L) The effect of SNORD72 on the mRNA stability of ID2. *: P < 0.05; **: P < 0.01; ***: P < 0.001.