Research Paper Volume 12, Issue 5 pp 4558—4572

Long non-coding RNA RHPN1-AS1 promotes tumorigenesis and metastasis of ovarian cancer by acting as a ceRNA against miR-596 and upregulating LETM1

Figure 1. RHPN1-AS1 is overexpressed in EOC tissues and cells and predicts poor prognosis. (A) The heat map of lncRNAs with the largest difference between the three pairs of EOC and control tissues (P < 0.05). (B, C) RHPN1-AS1 expression in EOC specimens, control tissues, and EOC cell lines. (DF) Association between RHPN1-AS1 expression and survival by Kaplan–Meier analysis. (G) m6A methylation level of RHPN1-AS1 in EOC cells and HOSEPIC cells determined by MeRIP-qPCR. (H) Changes in m6A-modified RHPN1-AS1 levels after METTL3 sectioning in HEY cells. (I) METTL3 and RHPN1-AS1 expressions in sh-METTL3-HEY cells. (J) RHPN1-AS1 stability was decreased in HEY cells with METTL3 knockdown. (K) Correlation between RHPN1-AS1 and METTL3 expression in EOC tissues.*P<0.05, **P<0.01.