Research Paper Volume 12, Issue 6 pp 4953—4969

RNA sequencing analysis of monocrotaline-induced PAH reveals dysregulated chemokine and neuroactive ligand receptor pathways

Figure 1. Schematic workflow of RNA-seq and bioinformatics analysis. MCT-treatment rats were treated with MCT for 1, 2, 3 and 4 weeks, and then total RNA was isolated from control and MCT-treated rats. After cDNA library preparation and RNA-seq, the datasets were generated and submitted to bioinformatics analysis, including differential expression analysis, GO enrichment analysis, KEGG pathway enrichment analysis and clustering analysis.