Research Paper Volume 12, Issue 6 pp 5048—5070

Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma

Figure 6. CA9, CXCL9, and GIMAP7 regulate the expression level of FOXO1 in PAAD. PANC-1 and SW 1990 cells were transfected with indicated siRNA. Then, 24 hrs post-transfection, cells were harvested for RT-qPCR. The data shown were the mean values ± SD from three replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. After 48 h post-transfection, cells were harvested for Western Blot analysis. (AC) PANC-1 and SW 1990 cells transfected with Si-CA9 (A), Si-CXCL9 (B) and Si-GIMAP7 (C) were harvested for RT-qPCR. (DF) PANC-1 and SW 1990 cells transfected with Si-CA9 (D), Si-CXCL9 (E) and Si-GIMAP7 (F) were harvested for RT-qPCR and western blotting. (G) PANC-1 cells transfected with Si-CA9 (C), Si-CXCL9 (E), or Si-GIMAP7 (G) were harvested for RT-qPCR.