Research Paper Volume 12, Issue 6 pp 5091—5120
Time-dependent interactions of blood platelets and cancer cells, accompanied by extramedullary hematopoiesis, lead to increased platelet activation and reactivity in a mouse orthotopic model of breast cancer – implications for pulmonary and liver metastasis
Figure 13. Representative dot plots and histograms showing gating strategy for the detection of platelet-cancer cells aggregates in mice injected with 4T1 cells or saline. The parent gate was set to the cancer cells based on FSC/SSC physical parameters (gate P1, in blue). Next, within this gated population, platelet-cancer cells aggregates were identified according to the surface presence of both CD41/61 (a unique antigen for platelets) (gate P2, in green) and CD24 (marker for cancer cells) (gate P3, corrected on the isotype binding, in red) in blood samples taken from mice five weeks after the injection with saline (A) or 4T1 breast cancer cells (B).