Research Paper Volume 12, Issue 6 pp 5091—5120

Time-dependent interactions of blood platelets and cancer cells, accompanied by extramedullary hematopoiesis, lead to increased platelet activation and reactivity in a mouse orthotopic model of breast cancer – implications for pulmonary and liver metastasis

Figure 14. In vitro activation of whole blood platelets incubated with 4T1 cells. Results are presented as median (horizontal line) and interquartile range (box) (n = 8). The expressions of P-selectin (CD62P) (A), the active form of GPIIb/IIIa complex (B) and the binding of endogenous vWF (C) and endogenous fibrinogen (Fg) (D) on platelets was measured using flow cytometry in non-fixed ‘washed blood’ incubated with 4T1-cancer cells or saline. Results are expressed as the percent fraction of the CD41/61-positive platelets. More experimental details are given in the Materials and methods section. The statistical significance of differences, estimated with the Mann-Whitney U-test, was: P-selectin, P1,α < 0.001, 4T1 >saline; active form of GPIIb/IIIa, P1,α < 0.001, 4T1 > saline; vWF, P1,α < 0.01, 4T1 > saline; Fg, P1,α < 0.05, 4T1 > saline.