Figure 15. The effect of the inhibition of GPIIb/IIIa complex and GPIb on the formation of platelet-4T1 aggregates in washed blood samples. Results are presented as median (horizontal line) and interquartile range (box) (n = 8). Aliquots of washed blood, preincubated with blocking antibodies anti-GPIIb/IIIa (n = 8), anti-GPIbα or saline, were mixed with CellTracker-labeled 4T1 cancer cells or saline. Results are expressed as the percent fraction of CellTracker/CD41/61-positive cells (4T1-platelet aggregates). More experimental details are given in the Materials and methods section. The statistical significance of the differences, estimated with the Kruskal-Wallis’ test and the post hoc multiple comparisons Conover-Inman test, was: P1,α < 0.001, saline < 4T1saline = 4T1GPIb = 4T1GPIIb/IIIa.