Figure 3. Representative images of immunochemistry detection of the extramedullary hematopoiesis foci in liver of mice injected with 4T1 cancer cells. Extramedullary hematopoiesis was diagnosed by immunohistochemistry staining at time interval t5 in slides from liver isolated during necropsy in mice injected with 4T1-cancer cells. The expressions of hematopoietic markers: CD117 (erythroid marker) (A, B), MPO (granulopoietic marker) (C, D) and FVIII (hematopoietic markers for megakaryocyte) (E, F) were detected. Additional hematoxylin staining was applied. Magnification of 100X (A, C) and 400X (B, D, E, F). Extramedullary hematopoiesis foci are marked with white arrows. More experimental details are given in the Materials and methods section.